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pcag archon1 kgc egfp er2 wpre (Addgene inc)

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Addgene inc pcag archon1 kgc egfp er2 wpre
Pcag Archon1 Kgc Egfp Er2 Wpre, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcag+archon1+kgc+egfp+er2+wpre/pCAG-Archon1-KGC-EGFP-ER2-WPRE+(Plasmid+%23108423)/pm40295764-267-11-12
Average 93 stars, based on 10 article reviews

pcag archon1 kgc egfp er2 wpre - by Bioz Stars, 2026-09

93/100 stars

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Derivative Assay:

Article Title: Expanding the family of genetically encoded voltage indicators with a candidate Heliorhodopsin exhibiting near-infrared fluorescence
Article Snippet: The sequences for targeting and endoplasmic reticulum export motifs (TSX3ER2) and Citrine were derived from MPC020: CamKII CMV_NovArch_citrine, which was a gift from Adam Cohen (Addgene plasmid # 153193) ( ). .. The pCAG backbone was derived from pCAG-Archon1-KGC-EGFP-ER2-WPRE, which was a gift from Edward Boyden (Addgene plasmid # 108423) ( ). .. pBAD-Helios-NT-6xHis was transformed into chemically competent E. coli cells (NEB 5-alpha, NEB) as per the manufacturer’s protocol.

Plasmid Preparation:

Clone Assay:

Article Title: Monitoring in real time and far-red imaging of H 2 O 2 dynamics with subcellular resolution.
Article Snippet: Justin Daho Lee , Amanda Nguyen , Chelsea E. Gibbs, Zheyu Ruby Jin, Yuxuan Wang, Aida Moghadasi, Sarah J.. Wait, Hojun Choi, Kira M. Evitts, Anthony Asencio, Samantha B. Bremner , Shani Zuniga, Vedant Chavan, Inez K. A. Pranoto , C. Andrew Williams, Annette Smith, Farid Moussavi-Harami , Michael Regnier, David Baker , Jessica E. Young, David L. Mack , Elizabeth Nance , Patrick M. Boyle & Andre Berndt

Article Title: Machine Learning Ensemble Directed Engineering of Genetically Encoded Fluorescent Calcium Indicators
Article Snippet: Sanger sequencing (Genewiz; Seattle, WA) of the isolated plasmid DNA was used to confirm the presence of the intended mutation. .. Genes encoding the GCaMP variants were cloned into a CAG-driven backbone, pCAG-Archon1-KGC-EGFP-ER2-WPRE (Addgene; #108423), using Gibson assembly (New England Biolabs; E2621L). .. All subsequences were verified with Sanger sequencing (Genewiz; Seattle, Wa).

Article Title: Far-red and sensitive sensor for monitoring real time H 2 O 2 dynamics with subcellular resolution and in multi-parametric imaging applications
Article Snippet: Sanger sequencing (Genewiz; Seattle, WA) or Whole-plasmid nanopore sequencing (Plasmidsarus; Eugene, OR) of the isolated plasmid DNA was used to confirm the presence of the intended mutation. .. Genes encoding the final variants were cloned into a CAG-driven backbone, pCAG-Archon1-KGC-EGFP-ER2-WPRE (Addgene; #108423), using the methods above. .. All subsequences were verified with Sanger sequencing (Genewiz; Seattle, WA) or Whole-plasmid nanopore sequencing (Plasmidsarus; Eugene, OR).

Article Title: Structure-guided engineering of a fast genetically encoded sensor for real-time H 2 O 2 monitoring
Article Snippet: Sanger sequencing (Genewiz; Seattle, WA) or Whole plasmid nanopore sequencing (Plasmidsarus; Eugene, OR) of the isolated plasmid DNA was used to confirm the presence of the intended mutation. .. Genes encoding the final variants were cloned into a CAG-driven backbone, pCAG-Archon1-KGC-EGFP-ER2-WPRE (Addgene; #108423), using the methods above. ..

Article Title: Ultra-fast genetically encoded sensor for precise real-time monitoring of physiological and pathophysiological peroxide dynamics
Article Snippet: Sanger sequencing (Genewiz; Seattle, WA) or Whole plasmid nanopore sequencing (Plasmidsarus; Eugene, OR) of the isolated plasmid DNA was used to con rm the presence of the intended mutation. .. Genes encoding the nal variants were cloned into a CAG-driven backbone, pCAG-Archon1-KGC-EGFP-ER2-WPRE (Addgene; #108423), using the methods above. ..

other:

Article Title: Far-red and sensitive sensor for monitoring real time H2O2 dynamics with subcellular resolution and in multi-parametric imaging applications.
Article Snippet: Methods Molecular Biology oROS-HT variants were all cloned based on the pC1 plasmid backbone from pC1-HyPer-Red (Addgene ID: 48249).

Image Search Results

Fig. 1 Archon1 and nirButterfly features. (a) Structural schematic of Archon1. (b) The normalized emission spectrum of Archon1 (N ¼ 3 cells, mean ± SEM). The red shaded area denotes the transmission band of the 676/37 nm emission filter used for the epifluorescence side-by-side functional comparison experiments. (c) Structural schematic of nirButterfly. (d) Same as in (b), with the normalized emission spectrum of miRFP670,22 the donor fluorescent protein of nirButterfly.

Journal: Neurophotonics

Article Title: Characterization of two near-infrared genetically encoded voltage indicators

doi: 10.1117/1.nph.11.2.024201

Figure Lengend Snippet: Fig. 1 Archon1 and nirButterfly features. (a) Structural schematic of Archon1. (b) The normalized emission spectrum of Archon1 (N ¼ 3 cells, mean ± SEM). The red shaded area denotes the transmission band of the 676/37 nm emission filter used for the epifluorescence side-by-side functional comparison experiments. (c) Structural schematic of nirButterfly. (d) Same as in (b), with the normalized emission spectrum of miRFP670,22 the donor fluorescent protein of nirButterfly.

Article Snippet: AAV vector plasmids, pAAVCaMK2A-nirButterfly and pAAV-CaMK2A-Archon1, were produced by subcloning nirButterfly and Archon1, respectively, into the pAAV-CW3SL vector in place of EGFP (Addgene #61463) to express the GEVIs under identical virus plasmid backbones.

Techniques: Transmission Assay, Functional Assay, Comparison

Fig. 2 Brightness comparison between nirButterfly and Archon1 in HEK cells and cultured neurons. (a) Representative images of HEK293 cells co-expressing the GEVI (NIR channel, top) and EGFP (below) after being side-by-side transfected with pCAG-nirButterfly-IRES-EGFP (left) or pCAG-Archon1-IRES-EGFP (right). For visualization, image gain is enhanced by 1.5× for the EGFP channel of nirButterfly and 5× for the near-infrared channel of Archon1. Scale bar ¼ 10 μm (b) Absolute fluorescence intensities (red-green bars) in the near-infrared and EGFP channels for nirButterfly and Archon1 (N ¼ 26 FOVs for nirButterfly; 24 for Archon1). EGFP-normalized fluorescence intensities (black bars). (c) Cultured neurons were side-by-side transduced with AAV1.CaMK2A-nirButterfly or AAV1.CaMK2A-Archon1. Images are shown at the same display gain, both without supplemental chromophores. The Archon1 inset shows the same FOV with the image gain 4× enhanced. Arrows indicate expression aggregates. Scale bar ¼ 20 μm. (d) Absolute fluorescence intensities for cultures transduced with nirButterfly (N ¼ 10 FOVs for nirButterfly with biliverdin; 5 for nirButterfly without biliverdin) and Archon1 (N ¼ 10 for Archon1 with all-trans-retinal; N ¼ 5 for Archon1 without all-trans-retinal), both with and without supple- ments of their respective chromophores (biliverdin for nirButterfly, and all-trans-retinal for Archon1). Data presented in median ± SEM; *** = p < 0.001; individual data points shown for N ¼< 10.

Journal: Neurophotonics

Article Title: Characterization of two near-infrared genetically encoded voltage indicators

doi: 10.1117/1.nph.11.2.024201

Figure Lengend Snippet: Fig. 2 Brightness comparison between nirButterfly and Archon1 in HEK cells and cultured neurons. (a) Representative images of HEK293 cells co-expressing the GEVI (NIR channel, top) and EGFP (below) after being side-by-side transfected with pCAG-nirButterfly-IRES-EGFP (left) or pCAG-Archon1-IRES-EGFP (right). For visualization, image gain is enhanced by 1.5× for the EGFP channel of nirButterfly and 5× for the near-infrared channel of Archon1. Scale bar ¼ 10 μm (b) Absolute fluorescence intensities (red-green bars) in the near-infrared and EGFP channels for nirButterfly and Archon1 (N ¼ 26 FOVs for nirButterfly; 24 for Archon1). EGFP-normalized fluorescence intensities (black bars). (c) Cultured neurons were side-by-side transduced with AAV1.CaMK2A-nirButterfly or AAV1.CaMK2A-Archon1. Images are shown at the same display gain, both without supplemental chromophores. The Archon1 inset shows the same FOV with the image gain 4× enhanced. Arrows indicate expression aggregates. Scale bar ¼ 20 μm. (d) Absolute fluorescence intensities for cultures transduced with nirButterfly (N ¼ 10 FOVs for nirButterfly with biliverdin; 5 for nirButterfly without biliverdin) and Archon1 (N ¼ 10 for Archon1 with all-trans-retinal; N ¼ 5 for Archon1 without all-trans-retinal), both with and without supple- ments of their respective chromophores (biliverdin for nirButterfly, and all-trans-retinal for Archon1). Data presented in median ± SEM; *** = p < 0.001; individual data points shown for N ¼< 10.

Techniques: Comparison, Cell Culture, Expressing, Transfection, Fluorescence, Transduction

Fig. 3 GEVI sensitivity and kinetic comparisons in acute brain slices. (a) (left) AAV-transduced nirButterfly expression in hippocampal pyramidal neurons, and (right) representative population response in CA3 following Schaffer collateral stimulation under standard ACSF, Gabazine- ACSF, and post-Gabazine recovery conditions (10-trial average). The dotted circle outlines the region of photon sampling for the photodiodes. The position of the stimulation electrode was outside the imaging field of view. (b) Same as in (a) for Archon1. Traces were acquired with five times higher illumination intensity. (c) GEVI sensitivity was measured as the population peak response amplitude normalized to baseline fluorescence for following 1-pulse (p ¼ 0.024, two- sample t-test with unequal variance) and five-pulse synaptic stimulation (p ¼ 0.002, two-sample t-test with unequal variance). (d) GEVI kinetic properties are measured as the decay time con- stants following 1-pulse (p ¼ 0.002, two-sample t-test with unequal variance) and 5-pulse synaptic stimulation (p ¼ 0.003, two-sample t-test with unequal variance). nirButterfly: N ¼ 8 slices from 3 mice; Archon1: N ¼ 11 slices from 3 mice. Data presented in median ± SEM; * = p < 0.05. ** = p < 0.01.

Journal: Neurophotonics

Article Title: Characterization of two near-infrared genetically encoded voltage indicators

doi: 10.1117/1.nph.11.2.024201

Figure Lengend Snippet: Fig. 3 GEVI sensitivity and kinetic comparisons in acute brain slices. (a) (left) AAV-transduced nirButterfly expression in hippocampal pyramidal neurons, and (right) representative population response in CA3 following Schaffer collateral stimulation under standard ACSF, Gabazine- ACSF, and post-Gabazine recovery conditions (10-trial average). The dotted circle outlines the region of photon sampling for the photodiodes. The position of the stimulation electrode was outside the imaging field of view. (b) Same as in (a) for Archon1. Traces were acquired with five times higher illumination intensity. (c) GEVI sensitivity was measured as the population peak response amplitude normalized to baseline fluorescence for following 1-pulse (p ¼ 0.024, two- sample t-test with unequal variance) and five-pulse synaptic stimulation (p ¼ 0.002, two-sample t-test with unequal variance). (d) GEVI kinetic properties are measured as the decay time con- stants following 1-pulse (p ¼ 0.002, two-sample t-test with unequal variance) and 5-pulse synaptic stimulation (p ¼ 0.003, two-sample t-test with unequal variance). nirButterfly: N ¼ 8 slices from 3 mice; Archon1: N ¼ 11 slices from 3 mice. Data presented in median ± SEM; * = p < 0.05. ** = p < 0.01.

Techniques: Expressing, Sampling, Imaging, Fluorescence

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pcag archon1 kgc egfp er2 wpre (Addgene inc)

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